ReMEDy2

Phase 2/3 Adaptive Design, Randomized Double-blind Placebo-controlled Study to Evaluate the Safety and Efficacy of DM199 for the Treatment of Acute Ischemic Stroke (ReMEDy2 Trial): A trial to evaluate the safety and efficacy of DM199 (rinvecalinase alfa) in participants with moderate stroke severity who

1.  Within 24 hours of disabling Acute Ischemic Stroke (AIS) onset

2.  Has a small or medium vessel occlusion (No LVO, No EVT, no brainstem or cerebellum)

3.  Ongoing significant deficits - persistent NIHSS 5-15 after 6-24 H

4.  Not hypotensive (sBP >100)

5.  Not on ACE-I (or willing to switch) and no angioedema history

🔵 Treatment:

• Participants with AIS will be randomized 1:1 to DM199 or placebo.

•  Day 1:

o   a single IV infusion: 0.5 µg/kg of DM199 (not to exceed 50 µg) in normal saline or placebo (50 mL normal saline total) IV infusion.

o   First SC injection of 3 µg/kg of DM199 or placebo SC at 2 hours (+10 hours) of IV infusion completion

•  Next 3 weeks: SC injection of 3 µg/kg of DM199 or placebo 2x per week until Day 21 (total of 7 SC doses).  Study to arrange support for injections at home or rehab after discharge – no need to stay in hospital.

💠 Treatment Duration: Treatments 2/week from day 1 to 21 (3 weeks). 

💠 Follow Up:   2/week until 3 weeks; day 30 (phone) and day 90 (in-person).

💠 Primary Objective: Stroke recovery, as defined by excellent functional outcomes on the Day 90 mRS (dichotomized; (0-1 vs. 2-6). 

Written consent with patient/SDM (requiring PI signature)

*For any study related questions call Dr. Swartz or Amanpreet (519-774-8785).

Inclusion Criteria

1. Participant is between 18 and 90 years of age inclusive.

2. Participant weight is 40 kg to 166 kg inclusive.

3. Participant to be randomized and treatment initiated within 24 hours of last known normal/AIS stroke onset.

4. Participant has NIHSS ≥5 and ≤15 at approximately the time of randomization. This criterion also applies to participants who meet the following conditions:

   1. • The participant initially presents with an NIHSS score below 5 but clinically worsens, including cases of progressing stroke/stroke-in-evolution, resulting in a subsequent persistent NIHSS score of ≥5 and ≤15; and 

      • Participant meets all other inclusion and exclusion criteria, including repeat brain imaging to rule out hemorrhagic transformation

5. Participant had a pre-morbid mRS score of 0 to 1 (mRS score prior to AIS) as stated by participant or participant's representative. 

6. If participant has received fibrinolytic treatment for AIS within 4.5 hours of last known normal/AIS stroke onset and at least 6 hours after completing fibrinolytic treatment, and the participant meets all the following criteria:

   1. • Participant's initial NIHSS score prior to fibrinolytics was ≤15; and

      • At least six hours after fibrinolytics, the participant has NIHSS score of ≥5 and ≤15 with a persistent deficit; and 

      • The participant's NIHSS score showed less than a 4-point improvement, or worsened, after receiving fibrinolytics; and

      • Participant meets all other inclusion and exclusion criteria including repeat brain imaging to rule out hemorrhagic transformation.

7. Participant and/or legally authorized representative is able to provide informed consent.

8. Participant is willing and able to comply with the study protocol, in the Investigator's judgment.

Exclusion Criteria

1. At screening, or with repeat imaging (see Inclusion 4 and 6), participant has imaging confirmed hemorrhagic stroke

2. Participant has image findings with symptomatic large vessel occlusion at one or more of the following locations: Intracranial carotid I/T/L or M1 segment MCA, vertebral or basilar artery (BA). 

3. Participant has large core of established infarction defined as ASPECTS 0-5

4. Participant has or will receive MT for their current AIS.

5. Participant has suspected or confirmed extracranial arterial dissection

6. Participant has imaging findings and/or symptoms consistent with a brain stem or cerebellar stroke. Posterior cerebral artery strokes without any associated brain stem or cerebellar involvement are allowable.

7. Participant has any recorded SBP < 100 mm HG or MAP <65 mm Hg; MAP = DBP + [1/3 (SBP - DBP)] (measured with noninvasive BP cuff type monitor) after stroke symptom onset and prior to randomization.

8. Participant is currently prescribed angiotensin-converting enzyme inhibitor (ACE) and is unable or unwilling to convert to another antihypertensive pharmacological treatment through Day 29 +1 day (8 days after last treatment).

9. Participant is currently prescribed an ACEi, and the last dose of the ACE inhibitor medication is reported to have been taken <24 hours before start of IV study drug infusion as stated by participant or participant's representative.

10. Participant has a history of clinically significant allergic reactions such as angioedema or anaphylaxis requiring hospitalization.

11. Participant has a diagnosis or suspected diagnosis of hereditary angioedema (HAE) or is taking or prescribed medications commonly used as prophylaxis/treatment of HAE, such as C1- esterase inhibitors (Cinryze, Berinert, Ruconest, Haegarda), Danazol, kallikrein inhibitors (Ecallantide, Berotralstat, Lanadelumab), Bradykinin B2 Receptor Antagonists (Icatibant), or other medication designed to influence the kallikrein-kinin system.

12. Life expectancy estimated at ≤1 year prior to enrollment.

13. Participant has clinical evidence of an active infection at the time of enrollment requiring parenteral treatment or hospitalization to monitor or manage the infection.

NOTE: Treatment of uncomplicated infections with oral antibiotics would not be an exclusion (example treatment of an uncomplicated urinary tract infection or sinus infections with oral antibiotics would not be an exclusion).

14. Participant has known alpha 1-antitrypsin deficiency (al-antitrypsin deficiency).

15. Participant is pregnant or nursing.

NOTE: Participants who agree to stop nursing may be considered for inclusion at the discretion of the Investigator.

16. Participants of child-bearing potential must agree to use medically acceptable contraceptive measures to prevent pregnancy. All participants of childbearing potential (defined as sexually mature participants who have had menses within the preceding 24 months and have not undergone permanent sterilization methods such as hysterectomy, bilateral oophorectomy, bilateral salpingectomy, etc.) must have a negative serum pregnancy test performed locally at screening. Participants of childbearing potential must agree not to attempt to become pregnant or undergo in vitro fertilization. If participating in sexual activity that could lead to pregnancy, participants must use 2 reliable methods (1 per partner is acceptable) of contraception simultaneously while receiving protocol-specified medication and during the study follow-up period.

Participants participating in sexual activity must agree to use, or for their partner to use highly effective birth control methods (those with a failure rate of less than 1% per year when used consistently and correctly) until they have completed the study (after the Day 90 visit). Such methods include:

• Combined (estrogen and progesterone containing) hormonal oral, intravaginal, or transdermal contraception associated with the inhibition of ovulation

• Progesterone-only oral, injectable, or implantable hormonal contraception associated with the inhibition of ovulation

• Intrauterine device (IUD)

• Intrauterine hormone-releasing system (IUS)

• Bilateral tubal occlusion

• Vasectomized partner

• Sexual abstinence

Participants who are not of reproductive potential (who have been postmenopausal for more than 24 consecutive months or have undergone hysterectomy, bilateral oophorectomy) are not required to use contraception.

Participants are prohibited from sperm donation.

NOTE: A negative serum pregnancy test will be documented during screening if a participant is of child-bearing potential.

17. Participant is currently participating in or has participated in a study using an investigational device or drug or received an investigational drug or investigational use of a licensed drug within 30 days prior to screening.

18. Participant does not have sufficient venous access for infusion of study treatment or blood sampling.

19. Participant is unable or unwilling to comply with protocol requirements, including assessments, tests, and follow-up visits.

20. Participant has any other medical condition which in the opinion of the Investigator will make participation medically unsafe or interfere with the study results.

Schedule of Activities: